Part:BBa_K3424022:Design

PhoBR Circuit

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2984
  Illegal SapI.rc site found at 535
  

Design Notes

When designing the system, make sure there are 4 nucleotides in between the terminator(BBa_K3424009) and the following promoter like pPhoA. The source link to this terminator also offers different sequences for assembling genetic circuits, if interested in using as well.

Source

https://static-content.springer.com/esm/art%3A10.1038%2Fs41589-018-0168-3/MediaObjects/41589_2018_168_MOESM1_ESM.pdf?fbclid=IwAR22C9bYtULbPECeq8TFaAhzM7D4_RDMQ9SoeSoqnM6OdHqtg_-hpK6GoXE

References

A dynamic model of the phosphate response system with synthetic promoters in Escherichia coli Cansu Uluşeker , Jesus Torres , José L. García , Martin M. Hanczyc , Juan Nogales , and Ozan Kahramanoğulları Artificial Life Conference Proceedings 2017 NO. 29, 412-419

Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3 Uluşeker C, Torres-Bacete J, García JL, Hanczyc MM, Nogales J, Kahramanoğulları O. Quantifying dynamic mechanisms of auto-regulation in Escherichia coli with synthetic promoter in response to varying external phosphate levels. Sci Rep. 2019 Feb 14;9(1):2076. doi: 10.1038/s41598-018-38223-w. PMID: 30765722; PMCID: PMC6376016.

Uluşeker C, Torres-Bacete J, García JL, Hanczyc MM, Nogales J, Kahramanoğulları O. Quantifying dynamic mechanisms of auto-regulation in Escherichia coli with synthetic promoter in response to varying external phosphate levels. Sci Rep. 2019 Feb 14;9(1):2076. doi: 10.1038/s41598-018-38223-w. PMID: 30765722; PMCID: PMC6376016.